Reduced motion artifacts and speed improvements in enhanced line-scanning fiber bundle endomicroscopy

Significance: Confocal laser scanning enables optical sectioning in fiber bundle endomicroscopy but limits the frame rate. To be able to better explore tissue morphology it is useful stitch sequentially acquired frames into a mosaic. However, low rates limit maximum probe translation speed. Line-scanning confocal provides higher rates, residual out-of-focus light degrades images. Subtraction based approaches can suppress this residue at expense of introducing motion artifacts. Aim: generate high rate images with improved sectioning, we develop high-speed subtraction method that only requires acquisition single camera frame. Approach: The rolling shutter CMOS acts as both aligned and offset detector slits required for subtraction-based enhancement. Two are formed on different regions camera, allowing simultaneously. Results: We confirm compared conventional line-scanning, particularly far from focus, show artifacts not introduced. demonstrate mosaicing up 240 Hz. Conclusion: High-speed optically sectioned using new approach leads rates.